supplementary materials from Comparative visual ecology of cephalopods from different habitats

2016-09-08T06:44:33Z (GMT) by Wen-Sung Chung N. Justin Marshall
Previous investigations of vision and visual pigment evolution in aquatic predators have focused on fish and crustacean, generally ignoring the cephalopods. Since the first cephalopod opsin was sequenced in late 1980s, we now have data on over 50 cephalopod opsins, prompting this functional and phylogenetic examination. Much of this data does not specifically examine the visual pigment spectral absorbance position (<i>λ</i><sub>max</sub>) relative to environment or lifestyle and cephalopod opsin functional adaptation and visual ecology remain largely unknown. Here we introduce a new protocol for photoreceptor microspectrophotometry (MSP) that overcomes the difficulty of bleaching the bistable visual pigment and that reveals eight coastal coleoid cephalopods to be monochromatic with <i>λ</i><sub>max</sub> varying from 484 to 505 nm. A combination of current MSP results, the <i>λ</i><sub>max</sub> values previously characterized using cephalopod retinal extracts (467–500 nm) and the corresponding opsin phylogenetic tree were used for systematic comparisons with an end goal of examining the adaptations of coleoid visual pigments to different light environments. Spectral tuning shifts are described in response to different modes of life and light conditions. A new spectral tuning model suggests that nine amino acid substitution sites may determine the direction and the magnitude of spectral shifts.