<p dir="ltr">SUMMARY</p><p dir="ltr">GSLab addresses the need for effective image analysis tools in fluorescence microscopy by providing an open-source platform that enhances traditional phasor analysis with advanced features. Key capabilities include machine learning-based clustering, real-time monitoring, and quantitative unmixing of fluorescent species. Designed for both commercial and custom systems, GSLab provides researchers with comprehensive lifetime and spectral phasor image analysis tools to tackle complex biological problems.</p><p dir="ltr">Tutorial dataset: <a href="https://doi.org/10.6084/m9.figshare.28067108" target="_blank">https://doi.org/10.6084/m9.figshare.28067108</a></p><p dir="ltr">Windows installer version: <a href="https://doi.org/10.6084/m9.figshare.28655276" target="_blank">https://doi.org/10.6084/m9.figshare.28655276</a></p><p dir="ltr">GitHub may contain newer version:</p><p dir="ltr">Landing page: <a href="https://alexvallmitjana.github.io/GSLab/" rel="noreferrer" target="_blank">https://alexvallmitjana.github.io/GSLab/</a></p><p dir="ltr">Source: <a href="https://github.com/AlexVallmitjana/GSLab" rel="noreferrer" target="_blank">https://github.com/AlexVallmitjana/GSLab</a></p><p dir="ltr">CITING</p><p dir="ltr">If you find this software valuable, please cite our paper in Bioinformatics:</p><p dir="ltr"><i>Vallmitjana A. et al. GSLab: Open-Source Platform for Advanced Phasor Analysis in Fluorescence Microscopy, </i><i>Bioinformatics (2025). </i><a href="https://doi.org/10.1093/bioinformatics/btaf162" rel="noreferrer" target="_blank"><i>https://doi.org/10.1093/bioinformatics/btaf162</i></a></p>
Funding
Development of a fast scanning, extended field-of-view multiphoton microscope for clinical skin imaging
National Institute of Biomedical Imaging and Bioengineering