ppat.1008660.s002.pptx (691.32 kB)

Primary canine TNBC cells infected with a vaccinia virus-Copenhagen exhibit a reduced numbers of mininuclei compared to primary canine non-TNBC cells.

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posted on 19.10.2020, 17:33 by Béatrice Cambien, Kevin Lebrigand, Alberto Baeri, Nicolas Nottet, Catherine Compin, Audrey Lamit, Olivier Ferraris, Christophe N. Peyrefitte, Virginie Magnone, Jérôme Henriques, Laure-Emmanuelle Zaragosi, Sophie Giorgetti-Peraldi, Frédéric Bost, Marine Gautier-Isola, Roger Rezzonico, Pascal Barbry, Robert Barthel, Bernard Mari, Georges Vassaux

Non-TNBC (A, B) or TNBC (C, D) cells were infected with a vaccinia virus-Copenhagen strain recombinant in which GFP expression is driven by an immediate-early vaccinia virus promoter (MOI = 5). Three hours after infection, the cells were fixed and stained with propidium iodide (PI). A and B: GFP staining; C and D: propidium iodide staining; M: mininuclei. F, G, H, I.Non-TNBC (white bars) or TNBC (black bars) cells were infected with a vaccinia virus-Copenhagen strain recombinant in which GFP expression is driven by an immediate-early vaccinia virus promoter (MOI = 5). Three hours after infection, the cells were fixed and stained with propidium iodide (PI). The number of PI and GFP positive cells was determined. The percentage of GFP+ cells (F), the mean GFP fluorescence per cell (G), the percentage of mini-nuclei in GFP+ cells (H) and the number of mini-nuclei in nuclei-positive cells (I) are presented. (*** p < 0.001; ** p < 0.01; * p < 0.05; n.s: p > 0.05). The data were obtained from the analysis of 120 images obtained from 2 non-TNBC and 2 TNBC from 4 primary canine specimen. The detail of the samples used to obtain these data is listed in S5 Table.

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