Presentation1_Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries.zip (28.4 MB)
Download file

Presentation1_Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries.zip

Download (28.4 MB)
presentation
posted on 27.10.2021, 04:03 by Jidong Lang, Rongrong Zhu, Xue Sun, Siyu Zhu, Tianbao Li, Xiaoli Shi, Yanqi Sun, Zhou Yang, Weiwei Wang, Pingping Bing, Binsheng He, Geng Tian

Illumina is the leading sequencing platform in the next-generation sequencing (NGS) market globally. In recent years, MGI Tech has presented a series of new sequencers, including DNBSEQ-T7, MGISEQ-2000 and MGISEQ-200. As a complex application of NGS, cancer-detecting panels pose increasing demands for the high accuracy and sensitivity of sequencing and data analysis. In this study, we used the same capture DNA libraries constructed based on the Illumina protocol to evaluate the performance of the Illumina Nextseq500 and MGISEQ-2000 sequencing platforms. We found that the two platforms had high consistency in the results of hotspot mutation analysis; more importantly, we found that there was a significant loss of fragments in the 101–133 bp size range on the MGISEQ-2000 sequencing platform for Illumina libraries, but not for the capture DNA libraries prepared based on the MGISEQ protocol. This phenomenon may indicate fragment selection or low fragment ligation efficiency during the DNA circularization step, which is a unique step of the MGISEQ-2000 sequence platform. In conclusion, these different sequencing libraries and corresponding sequencing platforms are compatible with each other, but protocol and platform selection need to be carefully evaluated in combination with research purpose.

History

References