13058_2016_688_MOESM2_ESM.pptx (135.38 kB)

Additional file 2: of Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells

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posted on 12.03.2016, 05:00 by Annika Weigand, Anja Boos, Kereshmeh Tasbihi, Justus Beier, Paul Dalton, Michael Schrauder, Raymund Horch, Matthias Beckmann, Pamela Strissel, Reiner Strick
Gene expression of stem cell and hematopoietic markers in cell isolates. Mammary epithelial cell (MEC), adipose-derived stem cell (ADSC) and mesenchymal cell (MES) primary cell lines all from the same patients (four normal (NORMA1-4) and two breast tumor primary cell lines are indicated). a-f Expression results of positive stem cell markers CD105, CD73, CD90, CD36, CD29, CD44 and vimentin (VIM) are shown in four NORMA1-4 and two breast tumor (invasive inflammatory ductal carcinoma (IFDUC)1, triple-negative ductal carcinoma (TRIDUC)1) different primary cell lines using real-time PCR (*p ≤0.05). g-l Hematopoietic markers, CD14, CD19, CD34, CD45 and HLA-DR are shown from peripheral blood cells (PBC)/blood endothelial cells (BEC), four NORMA1-4 and two breast tumor (IFDUC1, TRIDUC1) primary cell lines using real-time PCR (*p ≤0.05). (PPTX 135 kb)