Evaluation of Degraded Human DNA Samples Using the Illumina® Global Screening Array

For this study, genomic DNA was experimentally degraded using ultraviolet C (UV-C) light at defined intervals up to 1.0 J/cm². Samples were quantified pre- and post-treatments and typed using GlobalFiler™ to confirm degradation. The STR profiles coupled with the Degradation Index (DI) from the quant method demonstrated that as the dosage increased, so too did the amount of degradation (quantified via visual pattern in the profile and increase in DI). Five (5) samples expressing specific degradation patterns based on STR results were selected for genome-wide SNP genotyping, in duplicate, on the iScan to evaluate the performance of the Infinium Global Screening Array in its ability to accurately type LCN degraded DNA sample types