supplementary data.pdf

GNPs Pretreatment triggers cell migration 

The facts revealing the interaction between the GNPs and MSCs is crucial to understand the potential effects of GNPs on biological systems from clinical point of view. The scratch wound assay is basically an in vitro model that depicts the in vivo incisional strategy 1. Cell migration ability is commonly used to assess the proliferation and migration rate of cells under certain stimuli 2. It also provides the important pathological and physiological insights like cell growth, inflammation, cancer metastasis and differentiation far beyond the healing of wound 3. GNPs treated hADMSCs exhibited the smooth proliferative potential as shown by the control cells (Fig 9A). Gold nanoparticles provide the protective, proliferative, and differential effects to mammalian cells depends upon the concentration, size, and morphology of GNPs 4. Plant mediated synthesis of GNPs also make them reliable for the proliferative potential of cells due to important bioactive components present in plant extract. Fig 9B depicted that GNPs treated hADMSCs covered the complete surface area of the well at day 3, as wound size became 0 in respective wells.