Rashmi Ira - D20024 Thesis Data: Chapter 3

In this study, we are presenting a novel method for designing synthetic microbial consortia with the aim of enhancing biohythane production via anaerobic digestion of organic wastes. The consortia design follows the complementarity principle by combining the natural microbial communities of diverse organic wastes to treat a wide range of substrates. This process includes the selective heat-pretreatment of the inoculum components to maximize efficiency, minimize energy consumption, and anaerobic acclimatization with organic load shock. 16S rRNA amplicon-based metagenomic sequencing was performed to understand the microbial community dynamics for an original waste sample to the final consortia designing to assess the structure of the microbial community and its shift during the mixing, heat pre-treatment and acclimatization.16S rRNA amplicon-based metagenomic sequencing was performed to understand the microbial community dynamics for original waste sample to the final consortia designing to assess the structure of the microbial community and its shift during the mixing, heat pre-treatment and acclimatization.

This data is associated with the microbial communities' metabolic and ecological functional traits, which were identified using FAPROTAX 1.2.10. (Functional Annotation of Prokaryotic Taxa) database.