QIIME2_RefDB_development.zip

Description of the complete workflow to develop a QIIME2-formatted reference database in order to carry out taxonomic assignment of amplicon sequencing data. The workflow detailed here describe the development of plant reference databases dedicated to the ITS2 and rbcL barcode sequences.

This procedure can be applied for any dataset imported from NCBI, which means that the presented methodology can be applied to develop reference databases for any barcode sequence and any domain of life. Reference sequence and taxonomy files are provided in a QIIME2 format (.qza files), but also in a standard format (.fasta and .tsv files) so that developed reference databases can be used outside of QIIME2 if desired.