Prominent Reaction to Tissue Factor Antibody in Hemal Node of Egyptian Water Buffalo (Bubalus bubalis)
Objective: Tissue Factor (TF) histology is an essential prognosticator of hemostasis and outcomes in local thrombus. Fresh tissue testing for pathologist evaluation is the best loyal technique for histology categorization, in spite of this, no literature in histology allude to the characteristics and the distribution of TF in hemal node of water buffalo species. In this research paper, author proposes an original approach to predicting cellular allocation to TF antibody in hemal node.
Materials and Methods: Over the branches of iliac arteries of the abdominal aorta and superficial temporal arteries of temporal region, samples were validated on a dataset comprising 7-12 fresh hemal nodes per healthy water buffalo. Samples were fixed in 10% neutral buffered formalin, sections were organized and stained for routine histological interpretations, and anti-TF antibody was utilized for immunofluorescence examination.
Results: Hemal node held a small peanut size. It was bordered with a connective tissue capsule fashioned up chiefly of collagen fibers along with few reticular fibers, and smooth muscle cells. Inside hemal node parenchyma, lymphoid follicles and blood sinusoids were spontaneously scattered. Immunofluorescence staining highlighted the positive TF expressions to a population of cells homing the capsule, trabeculae, and blood sinusoids. Whereas a few positive TF expression remained demarking the bounds of lymphoid follicles. Distribution of positive TF cells gained a significant area % between all parts of hemal node.
Conclusion: Hopefully, this research will provide a helpful reference for diagnosticians to monitor any morphological alterations in hemal node structure and TF locality.