Assembly and annotation of pale eelpout (Lycodes pallidus) genome

A chromosome-level genome assembly of pale eelpout (Lycodes pallidus) was created by integrating Illumina, PacBio circular consensus, and Hi-C sequencing technologies (BioProject accession number: PRJNA1231400). The final assembly spans a length of 753,345,134 bp with a scafford N50 of 28.58 Mb and a BUSCO completeness of 99.3%, indicating a high degree of completeness. Here, we present assembled and annotated results, including five files: (1) the final assembly of the genome (fLycpall_final_assembly.fa); (2) masked assembly sequence (fLycpall_final.masked.fa) produced by RepeatModeler2 v2.0.6 and RepeatMasker v4.1.7-p1; (3) General Feature Format file (fLycpall_final_braker3.gff3) and (4) protein sequence (fLycpall_final_braker3.aa) produced by BRAKER3 v3.0.7.6; and (5) functional annotation of genes (fLycpall_final_emapper.annotations) produced by eggNOG-mapper v2.1.12 combined with the eggNOG database (5.0.2). The BRAKER3 was empolyed by integrating evidence from RNA-seq reads, which were mapped to indexed soft-masked assembly using HISAT2 v2.2.1, and protein data from the vertebrate OrthoDB with GeneMark-ETP and AUGUSTUS.