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posted on 2025-04-24, 19:40 authored by Stefano Berri, Jordan H Boyle, Manlio TassieriManlio Tassieri, Ian A. Hope, Netta Cohen

Overview and purpose:


This database was picked to provide example footage recorded under varying conditions of lighting, focus and environment and

is especially suitable for computer vision student projects. While the footage is primarily of planar locomotion, slight head lifting and examples of the body crossing over are observed.


Notes:

Single animal movies

Spatial resolution: vga

Sampling rate: 25 Hz

Tracking: The microscope stage is moved to keep the worm within view


Technical limitations/computer vision challenges:

- The longer movies include multiple examples of body coiling and self-obstruction during turns (see e.g. file "08.04.22_N2_dialysis1.wmv").

- The tracking occasionally fails so the entire animal is not kept in view.

- Interaction with objects in the enviroment (microbeads, dirt)


All individuals are young adults with a body length of approximately 1mm.


filename description duration (min:sec)


N2 (wildtype)


07.07.24_N2_gel_smallbeed.avi example of interaction with bead 00:12

07.08.10_N2_agar_0500.mpg on 2.0% agar surface (forward+steering) 00:30

08.04.22_N2_dialysis1.wmv on dialysis membrane 06:25

08.09.03_N2_gel00_1.wmv in 1.00% gelatin in M9 17:11 (footage starts around 00:32)

08.10.21_N2_gs050_1.wmv in 0.50% gelatin in M9

08.10.21_N2_gs075_1.wmv in 0.75% gelatin in M9


UNC30


08.08.11_unc-30_agar_1130.mpg on 2.0% agar 00:39

08.08.15_unc-30_gel00_0149.mov in M9 buffer 01:00

08.08.20_unc-30_gel20_0255.mpg in 2.0% gelatin in M9 buffer 00:54


License: CC-BY

Attribution: Berri, Stefano, Jordan H. Boyle, Manlio Tassieri, Ian A. Hope, and Netta Cohen. "Forward locomotion of the nematode C. elegans is achieved through modulation of a single gait." HFSP journal 3, no. 3 (2009): 186-193. https://doi.org/10.2976/1.3082260

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