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Video S1 - Ligation of Signal Inhibitory Receptor on Leukocytes-1 Suppresses the Release of Neutrophil Extracellular Traps in Systemic Lupus Erythematosus

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posted on 18.10.2013, 03:46 by Kristof Van Avondt, Ruth Fritsch-Stork, Ronald H. W. M. Derksen, Linde Meyaard

NETs are released by healthy neutrophils in response to SLE plasma. Time lapse of NET formation (related to Fig 4A in which individual frames are shown) shows that NETs detected with Sytox Green, a cell-impermeable, DNA-specific dye, are released when neutrophils are exposed to SLE plasma. Using fluorescence microscopy neutrophils (blue – labelled with Hoechst 33342) were visualized releasing NET-DNA (green - dyed with Sytox Green) within 3 h.