Video_S1.mov (1.96 MB)
Video S1 - ALS-Associated TDP-43 Induces Endoplasmic Reticulum Stress, Which Drives Cytoplasmic TDP-43 Accumulation and Stress Granule Formation
media
posted on 2013-11-29, 03:16 authored by Adam K. Walker, Kai Y. Soo, Vinod Sundaramoorthy, Sonam Parakh, Yi Ma, Manal A. Farg, Robyn H. Wallace, Peter J. Crouch, Bradley J. Turner, Malcolm K. Horne, Julie D. AtkinAssociation of 218–414 TDP-43-mCherry inclusions with the Golgi apparatus. Neuro2a cells were transfected with the 218–414 TDP-43-mCherry construct, and treated with 10 µM MG132 for 24 h starting at 24 h post-transfection. Cells were processed for immunocytochemistry against GM130 (green) and serial z-stack images were taken at 0.50 µm intervals using confocal microscopy and post-processed using Olympus Fluoview v2.0 software. mCherry fluorescence is shown in red and nuclei (Hoechst dye) are shown in blue. Scale bar represents 10 µm.
(MOV)
