Additional_File_13_Video_8.mov (10.98 MB)
Video 8: Neuronal activity traces of representative neurons.
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posted on 2018-05-10, 09:26 authored by Bianca Migliori, Malika S. Datta, Christophe Dupre, Mehmet C. Apak, Shoh Asano, Ruixuan Gao, Edward S. Boyden, Ola Hermanson, Rafael Yuste, Raju TomerA visualization of the neuronal traces shown in Figure 7b of the related publication; presenting rapid volumetric calcium imaging of highly motile Hydra.
An advantage of the LSTM imaging technique (see below for details) is the capacity for rapid volumetric live imaging of samples undergoing substantial non-isomorphic rearrangements in their body shape and cellular density, resulting in continuously changing local optical properties.
This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.
LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.
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