Additional_File_8_Video_3.mov (140.23 MB)
Video 3: High-resolution LSTM imaging of intact Thy1-eYFP mouse central nervous system.
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posted on 2018-05-10, 09:26 authored by Bianca Migliori, Malika S. Datta, Christophe Dupre, Mehmet C. Apak, Shoh Asano, Ruixuan Gao, Edward S. Boyden, Ola Hermanson, Rafael Yuste, Raju TomerThe bounding box for the entire sample is 11.8mm x 27.6 mm x 5.2 mm, and for the sub volume shown is 5.1mm x 3.1mm x 3.5mm. The raw data was down-sampled 2x2 fold (to make the volume rendering feasible) for the sub-volume rendering.
This video demonstrates how LSTM enables rapid high-resolution imaging of such large samples with uniform imaging quality across, even for a sample with highly curved surfaces.
This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.
LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.
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