Additional_File_4_Video_1.mp4 (81.06 MB)
Video 1: 3D model of LSTM implementation.
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posted on 2018-05-10, 09:26 authored by Bianca Migliori, Malika S. Datta, Christophe Dupre, Mehmet C. Apak, Shoh Asano, Ruixuan Gao, Edward S. Boyden, Ola Hermanson, Rafael Yuste, Raju TomerThe 3D modelling and rendering was performed using Autodesk Inventor 2017, and the animation was performed using Autodesk Fusion 360 2017 and MATLAB. The components labelled are LS (laser source), collimator, ND (neural density) filter mount, iris, ETL (electrically tunable lens), slit, CL (cylindrical lens), Galvo scanner, SL (scan lens), iris and TL (tube lens).
The LSTM illumination and detection arms are implemented as rigid assemblies (using the caging system from Thorlabs), connected to a vertically mounted breadboard via x-y manual translation stages for finer adjustments.
This video relates to the development of the imaging technique LSTM: Light Sheet Theta Microscopy for rapid high-resolution imaging of large biological samples, an approach designed to overcome the limitations of 'standard' Light Sheet microscopy LSM in terms of lateral dimensions and imaging quality, while preserving the benefits of LSM in terms of imaging resolution, depth and speed.
LSTM uses two symmetrically-arranged oblique light-sheets, generated using independent illumination objectives, for rapid high-resolution imaging of large samples.
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