Supplemental videos S1-S3 for "Beating the Heart Failure Odds: Long-Term Survival after Myocardial Ischemia in Juvenile Rainbow Trout"

Supplemental video 1. A sample heart from a coronary-ligated trout, demonstrating real-time perfusion with Microfil silicone compound of small vessels within a mesh-like network located on the surface of the bulbus arteriosus and the base of the ventricle, indicating the onset of revascularization following 10 days of coronary ligation (video at 4 times speed).

Supplemental video 2. Computed tomography image visualization of coronary revascularization in a coronary-ligated trout reveals a mesh-like network of small vessels located on the surface of the bulbus arteriosus. Also, note the presence of a ring of vessels at the base of the bulbus arteriosus, which is not visually apparent in supplementary video 1 as it is covered by adipose tissue. Additionally, some vessels may appear discontinuous due to the potential shrinkage of the Microfil compound during the dehydration process with ethanol, to which the heart was subjected for storage.

Supplemental video 3. A sample heart from a trout, recovered for 166 days since ligation surgery, demonstrating real-time perfusion with Microfil silicone compound in the coronary circulation. Note the presence of collateral vessels coursing alongside the bulbus arteriosus (video at 4 times speed).