Selective Breast Cancer Cell Capture, Culture, and Immunocytochemical Analysis Using Self-Assembled Magnetic Bead Patterns in a Microfluidic Chip
mediaposted on 04.05.2010, 00:00 by Venkataragavalu Sivagnanam, Bo Song, Caroline Vandevyver, Jean-Claude G. Bünzli, Martin A. M. Gijs
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Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted electrostatic self-assembly. Hereafter, they were grafted by exposure to a solution of 5D10 monoclonal antibodies (mAb) and fibronectin (FN), with the first being used for immunospecific cell capture and the latter being used for cell adhesion and growth. A solution of target MCF-7 cells mixed with Jurkat cells was brought inside the microchannel, leading to specific MCF-7 cell capture; the latter were then cultured and evidenced by cell immuno-luminescence.