Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission
mediaposted on 2015-12-17, 05:03 authored by Yang Liu, Rebecca Medda, Zheng Liu, Kornelia Galior, Kevin Yehl, Joachim P. Spatz, Elisabetta Ada Cavalcanti-Adam, Khalid Salaita
Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.
Impacttension fluorescence microscopymechanismprobeNanoparticle Tension Probes PatternedNanoscalearrayligand spacingblock copolymer micelle nanolithographyRGD ligandsstrategyrolenanoscaleForce TransmissionHereinmechanotransduction pathwaysIntegrin Clusteringsubstratefluorescence responseintegrin receptors