Myo et al. 2025. 3D rendering of SAEC ALI culture

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posted on 2025-03-03, 21:33 authored by Yu Par Aung Myo, Sarah V Camus, Margaret A.T. Freeberg, Rebecca L. Heise, Thomas ThatcherThomas Thatcher, Patricia Sime

Primary small airway epithelial cells were differentiated at the air-liquid interface as described. The cells were fixed with 4% PFA and then stained for Krt5 (basal cells, white), CC10 (club cells, red), acetylated tubulin (ciliated cells, green), and DAPI (nuclei, blue). The membrane was then cut from the insert and mounted. Z-stacks were photographed with the Zeiss LSM 700 confocal and Leica Stellaris confocal microscopes and combined into a 3D render.

Data from Myo et al., Protocol For Differentiating Primary Human Small Airway Epithelial Cells at the Air-liquid Interface, Am J. Physiology: Lung Cell Mol. Physiology. 2025.

Funding

Dr. Margaret AT Freeberg was supported in part by the Parker B. Francis Fellowship and the Pulmonary Fibrosis Foundation Scholars Program. Microscopy was performed at the VCU Massey Cancer Center Microscopy Core Facility which is supported, in part, with funding from NIH-NCI Cancer Center Support Grant P30CA016059. Dr. Rebecca L. Heise was supported by NHLBI R01HL164508.

Myo et al. 2025. 3D rendering of SAEC ALI culture

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