Jorgens et al., JCS 2016 Movie 2

Movie 2: High resolution electron tomography of desmosome cell junction. Electron tomogram of a 100 nm thin section imaged every 1° from 70° to 70°. The nominal setting of defocus was 1 micrometer, and the pixel size of the data corresponded to 1 nm. The ultrastructural composition of the desmosome junction is visualized. Filaments between electron dense plaques on adjacent cell membranes are observed to span a gap of ~ 30 nm. Cytoskeletal filaments are observed linking into the electron dense plaques.