Example of how to inoculate Drosophila with pathogens using a fine needle

2017-05-31T09:08:12Z (GMT) by Ben Longdon
How to prick Drosophila with a fine needle for inoculating with pathogens as described in Longdon et al 2015 PLOS Pathogens.

Using 0.0125 mm diameter stainless steel needle (26002–10, Fine Science Tools, CA, USA) bent ~0.25 mm from the end, held in a needle holder (D431, Watkins and Doncaster, UK).