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Example of how to inoculate Drosophila with pathogens using a fine needle
How to prick Drosophila with a fine needle for inoculating with pathogens as described in Longdon et al 2015 PLOS Pathogens.
Using 0.0125 mm diameter stainless steel needle (26002–10, Fine Science Tools, CA, USA) bent ~0.25 mm from the end, held in a needle holder (D431, Watkins and Doncaster, UK).