A representative video cropped from S2 Video shows pillar displacement as an <i>E. histolytica</i> trophozoite migrates on a fibronectin-coated micropillar array, incubated with <i>E. coli</i> O55.

<p>Time stamp (minutes: seconds). When exposed to <i>E. coli</i> O55, the trophozoite transitions to rapid, goal-directed movement without actively pulling on the micropillars or engaging in random exploration, resulting in noticeably smaller micropillar deflections. In the videos, the curved organisms filling the screen are actually shadows of <i>E. coli</i>, distributed at different heights, due to the non-confocal nature of live imaging. In reality, the number of bacteria directly in contact with <i>E. histolytica</i> trophozoites is limited.</p> <p>(AVI)</p>