ppat.1008738.s015.mov (17.7 MB)
Download file3D reconstruction of A. algerae spore from SBFSEM.
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posted on 2020-09-18, 17:49 authored by Pattana Jaroenlak, Michael Cammer, Alina Davydov, Joseph Sall, Mahrukh Usmani, Feng-Xia Liang, Damian C. Ekiert, Gira BhabhaRepresentative 3D reconstruction of an A. algerae spore. At the beginning of the video, slices through the spore are shown. Each color represents an individual organelle: exospore (orange), endospore (yellow), PT (blue), vacuole (red), posterior polaroplast (purple), and anchoring disc (green).
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harpoon-like invasion apparatusresults show7 timesAnncaliia algeraecargo movesPT extensionhost cellPT germination processLive-cell imagingPT extrusiongain entryblock-face scanning electron microscopy3- Dimensional organization20 timeshost cellsextracellular sporehuman-infecting microsporidian speciesEncephalitozoon hellemPT experiencesinfection apparatustube invasion machinery Microsporidiasingle-celled eukaryotic parasites
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