<i>Pseudomonas cepacia</i> lipase immobilized on Zn₂Al layered double hydroxides: Evaluation of different methods of immobilization for the kinetic resolution of (<i>R,S</i>)-1-phenylethanol

<p>The present work deals with the preparation and characterization of Zn₂Al layered double hydroxides (LDHs) intercalated with chloride and dodecyl sulfate ions and their use as supports for the immobilization of <i>Pseudomonas cepacia</i> lipase (LipPS). LipPS was immobilized on LDH by adsorption, <i>in situ</i> entrapment and delamination entrapment methods. The performance of free and immobilized LipPS in the transesterification of (<i>R,S</i>)-1-phenylethanol was compared. The immobilized derivatives prepared by adsorption gave the highest conversions (<i>c</i> = 50%), with an enantiomeric excess of the product (<i>ee_p</i>) greater than 99% and a high enantiomeric ratio (<i>E</i> > 200). For immobilization by <i>in situ</i> entrapment (coprecipitation), a conversion of 31.5% and <i>E</i> > 200 were obtained. For immobilization by delamination entrapment, a conversion of 36% and <i>E</i>= 155 were obtained. Although the best results were obtained by adsorption, these results show that the delamination entrapment method can also be used to prepare bioinorganic-enzyme materials based on LDH. Our work therefore increases the range of methods that can be used to immobilize lipases for use in the production of optically pure secondary alcohols.</p> <p></p>