pone.0277274.s003.docx (350.46 kB)
WISH shows cd9b is significantly decreased in cd9b KO embryos.
journal contribution
posted on 2022-11-10, 18:31 authored by Sarah Greaves, Katherine S. Marsay, Peter N. Monk, Henry Roehl, Lynda J. PartridgeA-C: Representative images of cd9b WISH at 36 hpf in (a) WT, (b) cd9bpg15 homozygous embryos and (c) cd9bis16 homozygous embryos. (a) cd9b can be seen in the neuromasts and primordium of the posterior lateral line in WT embryos (arrows), but is absent in cd9b mutants (b,c). n = minimum 3 imaged, 10 observed per genotype. Data from a single experiment.
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analysed clutch sizeknockout zebrafish pairsdiv >< pcd9 null animalstetraspanin cd9b playszebrafish egg numbers couldcd9b cd9 playsvitro knockout partnercd9 expressioneffects couldwildtype partnersolely duesignificantly reducedresults indicatemating behaviourlower percentagefertilisation usingfertilisation techniquesfertilisation ratesfertilisation defectexchanging eitheregg fusionefficient spermcourtship behaviours
