Time of exposure.

We tested the effect of the exposure time to CPC. To do so, IAV/WSN/33 was incubated with CPC at 0.1% for 2 minutes, 1 minute, or 30 seconds. Next, the virus was diluted and used to infect MDCK cells as previously described. After 48 hours of infection, the viral load was assessed by TCID50. We used a 2-minute treatment with SDS at 0.05% as a positive control and PBS solution as a negative control. No differences in the viral load were observed between the 2 minutes, 1 minute, or 30 seconds exposure.

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