The coding-complete genome sequence of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain isolated from an Iraqi patient was sequenced for the first-time using Illumina MiSeq technology. There was a D614G mutation in the spike protein-coding sequence. This report is valuable for better understanding the spread of the virus in Iraq.
<p dir="ltr">The goal of this work was to look into the CRISPR-Cas system in the genome of <a href="https://www.sciencedirect.com/topics/food-science/staphylococcus-aureus" target="_blank">Staphylococcus aureus</a>. To do this, local strains of <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/staphylococcus-aureus" target="_blank">Staphylococcus aureus</a> bacteria were isolated from several samples and examined for confirmation of Staphylococcus spp. detection. Using the BLAST tool, the partial sequencing of the 16S <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/rna-gene" target="_blank">rRNA gene</a> data was evaluated and compared to those in GenBank to determine the sequence variance. The sequence results were 9 samples classified as <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/staphylococcus-aureus" target="_blank">Staphylococcus aureus</a>, which were utilized to build a <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/phylogenetic-tree" target="_blank">phylogenetic tree</a> to assess the similarities and differences between local isolates and existing international isolates. The CRISPR-Cas were investigated in all isolates of <i>Staphylococcus</i> spp. (non-aureus <i>Staphylococcus</i> and <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/staphylococcus-aureus" target="_blank">Staphylococcus aureus</a>), the cas6 element was not detected in all investigated <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/bacterial-strain" target="_blank">bacterial strains</a>, while <i>SECR1</i> and <i>SECR</i>2 were detected in 18 and 12 species of <i>Staphylococcus</i> spp. respectively. Moreover 6 isolates of confirmed <a href="https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/staphylococcus-aureus" target="_blank">Staphylococcus aureus</a> were found to have <i>SECR1</i> and <i>SECR</i>2 elements. We concluded that the CRISPR-Cas system can be detected in <i>S. aureus</i> isolates from various local sources of samples, and that the appearance of the <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/clustered-regularly-interspaced-short-palindromic-repeat" target="_blank">CRISPR</a> system in <i>S. aureus</i> isolates is lower than the expected average for bacteria in general, implying that <a href="https://www.sciencedirect.com/topics/immunology-and-microbiology/clustered-regularly-interspaced-short-palindromic-repeat" target="_blank">CRISPR</a> loci or the CRISPR-Cas system in <i>S. aureus</i> may be <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/mobile-genetic-elements" target="_blank">mobile genetic elements</a>.<br></p>
