The Mitochondrial Lactate Oxidation Complex: Endpoint for carbohydrate carbon disposalRobert G. Leija1, Jose A. Arevalo1, Dianna Xing2, José Pablo Vázquez‐Medina2, and George A. Brooks1 1Exercise Physiology Laboratory, Department of Integrative Biology, University of California, Berkeley, CA 94720-3140 2Vazquez‐Medina Lab, Department of Integrative Biology, University of California, Berkeley, CA 10 94720‐3140
Supplemental Material (Figures S1, Figure S2, Movie S1, Movie S2, Table S1, Table S2)
Figure S1. Fixed cross‐sections from the skeletal muscle of mice (63x magnification) demonstrate no to low signal of the fluorescent probes used in to detect the mLOC as demonstrated in Figure 3.
Figure S2. Fixed sections (10 μm) of mouse gastrocnemius were visualized here at 100X magnification and assessed for colocalization of mMCT1 (AF Green 488), mPC1 (AF Red 594), and COXIV (AF Blue 405); the merged panel (top left) shows colocalization of mMCT1, mPC1 and COx.
Movie S1. Three-Dimensional Reconstruction illustrating a muscle fiber rotating about the horizontal axis. The color scheme was based on colocalization of mPC1, mMCT1, and COx (Gold) and sarcolemmal MCT1 (Green). Sections utilized were prepared in the same manner as two-dimensional images.
Movie S2. Three-Dimensional Reconstruction illustrating an orthogonal view of the mitochondrial reticulum . The color scheme was based on colocalization of mPC1, mMCT1, and COx (Gold) and sarcolemmal MCT1 (Green). Sections utilized were prepared in the same manner as two-dimensional images.
Table S1. List of antibodies utilized including the experiment and the associated dilution factor.
Table S2 Data S1. Label-Free Proteome: List of peptides detected from isolated mitochondrial reticulum. The samples were utilized to identify the constituents making up the mitochondrial lactate oxidation complex (mLOC).
