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Surface-Enhanced Raman Scattering:  A Structure-Specific Detection Method for Capillary Electrophoresis

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journal contribution
posted on 28.09.2000, 00:00 authored by Lin He, Michael J. Natan, Christine D. Keating
A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn geometry. The retention time obtained from SERS corresponds well with that from conventional UV−visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).