ac000583v_si_001.pdf (93.44 kB)
Surface-Enhanced Raman Scattering: A Structure-Specific Detection Method for Capillary Electrophoresis
journal contribution
posted on 2000-09-28, 00:00 authored by Lin He, Michael J. Natan, Christine D. KeatingA new approach to detecting capillary electrophoresis (CE)
eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described.
In this approach, CE-based separation of a mixture of
trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn
geometry. The retention time obtained from SERS corresponds well with that from conventional UV−visible
detection. Meanwhile, CE eluants are identified by their
characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for
CE. In addition, the ability to monitor SERS intensity
changes at molecule-specific frequencies makes selective
detection of individual analytes possible, even when
separation is incomplete. Finally, CE-SERS is evaluated
for separation of amino acids (tyrosine and tryptophan)
and environmental pollutants (chlorophenol mixtures).