Inactivation of Smad 4 Accelerates KrasG 12 D-Mediated Pancreatic Neoplasia

Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal human malignancies, with an overall 5-year survival rate of <5%. Genetic analysis of PDAC patient samples has shown that specific disease-associated mutations are correlated with histologically defined stages of neoplastic progression in the ductal epithelium. Activating mutations in KRAS are almost uniformly present in early-stage disease, with subsequent inactivating mutations in p16, p53 , and SMAD4 occurring in more advanced lesions. In this study, we have tested whether the loss of Smad4 would cooperate with an activating Kras mutation to promote progression to PDAC using the Pdx1-Cre transgenic system to activate Kras and delete Smad4 in all pancreatic lineages including the ductal epithelium. Analysis of double-mutant mice showed that loss of Smad4 significantly accelerated the progression of pancreatic intraepithelial neoplasias (mPanIN) and promoted a high incidence of intraductal papillary mucinous neoplasia and active fibrosis compared with Pdx1-Cre;Kras or Pdx1-Cre;Smad4 mice. Occasionally, double-mutant mice progressed to locally invasive PDAC with little evidence of metastases by 6 months of age and without the detectable loss of p53 or p16 expression or function. The loss of Smad4 only seemed to promote disease progression in the presence of the activated Kras allele because we observed no abnormal pathology within the pancreata of 23 Pdx1-Cre;Smad4 animals that were analyzed up to 8 months of age. This indicates that Smad4 is dispensable for normal pancreatic development but is critical for at least partial suppression of multiple Kras-dependent diseaseassociated phenotypes. [Cancer Res 2007;67(17):8121–30]


Introduction
Infiltrating pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer death in the United States.It is almost uniformly fatal, with an overall 5-year survival rate of <5% (1,2).The extremely poor prognosis associated with PDAC is primarily due to the advanced stage of disease at the time of clinical diagnosis and the refractory nature of PDAC to conventional chemotherapy and radiotherapy regimens.
Preinvasive neoplasias affecting the pancreatic ductal epithelium have been grouped into three major pathologic categories that include pancreatic intraepithelial neoplasias (PanIN), which are primarily observed in the small ducts, intraductal papillary mucinous neoplasms (IPMN), which occur in the main pancreatic duct or in branch ducts, and mucinous cystic neoplasms, where cystic lesions are lined by a mucin-producing, tall columnar epithelium with an associated ovarian-type stroma.
Histologic and molecular genetic characterization of ductal lesions before invasive PDAC has led to the development of a staged progression model wherein the presence of specific diseaseassociated mutations is correlated with defined histologic abnormalities in the ductal epithelium (3,4).Neoplasias representing PanIN range from PanIN-1A and PanIN-1B to PanIN-2 and PanIN-3, with each stage showing increased cellular and nuclear atypia (5,6).PanIN-3 is frequently found in association with invasive PDAC and likely represents a precursor lesion to invasive disease.
A number of mutations have been associated with specific PanIN stages, including the activation of the KRAS2 and HER-2/NEU genes and the loss of the tumor suppressor proteins CDKN2A/p16 INK4A , p53, DPC4/SMAD4, and BRCA2 (7).The presence of an activating mutation in the KRAS2 locus was detected in f40% of early PanIN-1A and PanIN-1B lesions and is found in virtually 100% of advanced PDAC cases (8)(9)(10)(11)(12).Analysis of mice where a Pdx1-Cre transgene was used to activate expression of a constitutively active allele of Kras (Kras G12D ) in the ductal epithelium showed the full spectrum of preinvasive PanIN-like lesions (mPanIN), which supports the model that Kras activation is a critical initiating step in progression to PDAC (13,14).Although loss of p16 INK4A tumor suppressor expression can be detected in PanIN-1A and PanIN-1B lesions, it is more frequently associated with PanIN-2, wherein the ductal epithelium exhibits nuclear atypia and papillary architecture (15).In invasive PDAC, more than 90% of cases show loss of p16 INK4A function (12,(16)(17)(18)(19).In mice, loss of either p16 Ink4A or p53 in conjunction with constitutive Kras activity promotes progression to PDAC, which indicates that both the retinoblastoma (Rb) and p53 pathways are involved in suppression of PDAC progression (20,21).However, the observation that p53 remains intact in a Pdx1-Cre;Kras G12D/+ ;p16 Ink4AÀ/À genetic background that progresses to PDAC and that p16 Ink4A remains intact in Pdx1-Cre;Kras G12D ;p53 À/À ductal epithelia with PDAC indicates that inactivation of both pathways may not be necessary for fully penetrant disease in the mouse model.
Finally, inactivating mutations in the gene encoding the downstream transforming growth factor h (TGF-h) effector protein, deleted in pancreatic cancer 4 (DPC4/SMAD4/MADH4), have been found in association with late stages of PDAC progression when there is evidence of carcinoma in situ and invasive disease in f55% of PDAC cases (22,23).The loss of DPC4/SMAD4 has also been associated with familial juvenile polyposis (24) and with f15% of colorectal tumors (25).In mice, haploinsufficiency of Smad4 was sufficient to initiate gastric polyp formation and progression to invasive carcinoma that was associated with loss of the wild-type Smad4 allele in older animals (26).In a number of human PDAC cell lines that lack SMAD4, TGFh signaling through the receptor-associated SMADs, SMAD2 and SMAD3, seems to be intact in that both proteins are serine phosphorylated and localized to the nucleus (27,28).It has been proposed that the loss of SMAD4 may abrogate TGFh-induced cell cycle arrest but maintain TGFh-dependent, SMAD4-independent signaling pathways that function in tumor promotion (29).
Mutational analyses of IPMN samples have revealed similar genetic abnormalities to what has been observed in PanIN lesions, with a high incidence of activating KRAS mutations and loss of p16 and p53 expression (30)(31)(32).Loss of SMAD4 is not typically found in association with IPMN (32,33).
In this study, we have tested whether the loss of Smad4 would cooperate with an activating Kras G12D mutation to promote progression to PDAC using the Pdx1-Cre transgenic system (34,35).Analysis of double-mutant mice showed that loss of Smad4 significantly accelerated the progression of mPanIN lesions, the loss of acinar tissue, and the appearance of active fibrosis compared with single-mutant Pdx1-Cre;Kras G12D/+ or Pdx1-Cre;Smad4 lox/lox mice.Pdx1-Cre;Smad4 lox/lox mice showed no gross histologic abnormalities in the pancreas up to 8 months of age.A high frequency of double-mutant mice (14 of 16) also exhibited IPMN-like lesions of the gastric type and displayed duct ectasia with an associated pancreatitis.Lesions resembling mucinous cystic neoplasm were not observed in any of the animals and there was no evidence of ovarian-type stroma as determined by histologic examination and by negative staining for progesterone and estrogen receptor.These results indicate that, in conjunction with Kras G12D , the loss of Smad4 may stimulate PDAC progression through multiple factors including accelerated fibrosis, enhanced acinar cell loss, and more rapid promotion of neoplastic changes resembling mPanIN and IPMN within the ductal epithelium.

Materials and Methods
Mouse strains.The Pdx1-Cre transgenic strain originally generated on an ICR background was kindly provided by Doug Melton (35) and was backcrossed to C57BL/6 for four generations.The LSL-Kras G12D/+ knock-in strain on a C57BL/6/129SvJae background was purchased from the Mouse Models of Human Cancer Consortium (National Cancer Institute-Frederick) and was backcrossed to C57BL/6 for five generations.The floxed Smad4 strain on a C57BL/6 background was a kind gift from Chu-Xia Deng (36).The genotypes Pdx1-Cre;Smad4 lox/+ and Kras G12D/+ ;Smad4 flox/+ were intercrossed to generate double-mutant mice (Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox ).Genomic and recombination screens were done by PCR (primer sequences are given in Supplementary Methods).
Histology and immunohistochemistry. Tissues were fixed either in 70% ethanol/10% neutral buffered formalin overnight or in 4% paraformaldehyde in PBS for 4 h, and embedded in paraffin.Routine H&E staining was done by the Tissue Procurement Core Facility at University of Alabama at Birmingham.For immunohistochemistry, 4-Am sections were deparaffinized in xylene and rehydrated in ethanol.Heat-induced epitope retrieval was done on all slides in Tris-EDTA buffer (10 mmol/L Tris base, 1 mmol/L EDTA solution, 0.05% Tween 20, pH 9.0) using a Russell Hobbs Pressure Cooker (Fisher Scientific) at high setting for 10 min.Slides were incubated with 3% hydrogen peroxide to block endogenous peroxidase activity and then incubated with avidin/biotin blocking reagent (Vector Laboratories).Tissue sections were blocked at room temperature for 1 h using 3% normal goat serum, 1.5% normal horse serum, 2% normal rabbit serum, or Vector M.O.M. Basic Kit (Vector Laboratories) according to the manufacturer's instructions.Primary staining was done at room temperature for 1 h, followed by 20-min incubation with biotinylated secondary antibodies.Vectastain Elite ABC Kit (Vector Laboratories), which contains SAperoxidase, was used for biotin labeling.DAB Substrate Kit (Vector Laboratories) was used for visualization of peroxidase activity.Hematoxylin counterstaining was then done.Slides were dehydrated in ethanols and xylene and mounted with VectaMount permanent mounting medium (Vector Laboratories).The primary antibodies used were Smad4 (B-8, Santa Cruz Biotechnology), pSmad2 (Abcam), CK19 (BA17, DakoCytomation), Ki-67 (Abcam and Novus Biologicals), Muc1 (Abcam), Muc2 (H-300, Santa Cruz Biotechnology), PR (Abcam), and ER (Abcam).Secondary antibodies were biotinylated goat anti-rabbit immunoglobulin G (IgG; Vector Laboratories), biotinylated horse anti-goat IgG (Vector Laboratories), and biotinylated anti-mouse IgG from Vector M.O.M. Basic Kit following the manufacturer's instruction.For Alcian blue staining, 4-Am sections were deparaffinized and rehydrated.Slides were stained with Alcian blue solution (1-g Alcian blue in 100-mL 3% glacial acetic acid, pH 2.5) for 30 min at room temperature.Counterstaining with 0.1% nuclear fast red solution (0.1-g nuclear fast red and 5-g ammonium sulfate in 100-mL dH 2 O) was done for 5 min.Slides were dehydrated and mounted.

Results
Loss of Smad4 accelerates development of mPanIN lesions induced by oncogenic Kras G12D .To determine whether the loss of Smad4 would cooperate with a constitutively active allele of Kras to promote progression of PDAC, we generated F1 progeny by crossing Pdx1-Cre;Smad4 lox/+ and Kras G12D/+ ;Smad4 flox/+ animals (Fig. 1A).Activation of the inducible Kras G12D allele occurs by Cre-mediated deletion of transcriptional and translational stop sequences that were inserted upstream of the first coding exon of the Kras locus that also contained a knocked-in G12D mutation (13,37).Genotyping of animals was done by PCR, whereas expression of activated Kras G12D within pancreatic tissue was assessed using a pull-down assay to quantify the level of Ras-GTP (Fig. 1A).Deletion of floxed sequences within the Smad4 locus results in the loss of exon 8 and generates a null Smad4 allele in all endocrine and exocrine cells due to expression of the Pdx1-Cre transgene in pancreatic progenitor cells (Fig. 1A; refs.[34][35][36].Double-mutant animals (Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox ) were generated at the expected Mendelian frequencies, indicating that Pdx1-Cre-mediated inactivation of Smad4 was not lethal.
compared with age-matched Pdx1-Cre;Kras G12D/+ mice (Fig. 1B  and C).Only 1 of 17 double-mutant animals lived beyond 33 weeks of age, which limited comparative analyses beyond this point.Due to the near-complete loss of acinar tissue, which was mediated in part through acinar-ductal metaplasia (Fig. 1D, left), accurate assessment of mPanIN was often difficult in older animals and was typed in younger double-mutant animals with less severe acinar loss.Low-power examination of H&E-stained double-mutant pancreata used for Fig. 1C is shown in Supplementary Fig. S1; mouse nos.490, 523, and 506, with mouse no.484 not being used for analysis due to extensive acinar cell loss.In addition, Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice commonly exhibited ductulo-insular complex involvement in both young and older animals (Table 1; Fig. 1D, right).
Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice exhibit IPMN-like lesions with duct ectasia and chronic pancreatitis.In the majority of both young and older Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice (14 of 16 moribund animals, Table 1), we observed cystic lesions resembling IPMN of the gastric/foveolar subtype that occurred with or without evidence of papillae (Fig. 2G-L; refs.6,38,39).In most cases, the IPMN was an intraductal papillary mucinous adenoma (IPMA) that involved the branch ducts, which presented without significant architectural atypia and without papillae formation (Fig. 2G).IPMA was often accompanied by pancreatitis with duct ectasia and the occasional presence of inflammatory cells within the ducts (Supplementary Fig. S1, mouse no.456, and data not shown).In cases of IPMN with papillae formation (Fig. 2H-L), we observed rare instances where fibrovascular cores contained endocrine cells (Fig. 2H and I).A papilla composed of acinar cells was also noted in one case (Fig. 2J).Mucinous ducts stained positive for Muc1 and were Muc2 negative (Supplementary Fig. S2A).The surrounding stroma lacked expression of both progesterone and estrogen receptor (Supplementary Fig. S2B) and did not exhibit ''wavy'' appearing nuclei, which are a characteristic of stroma associated with mucinous cystic neoplasm in humans.The presence of cystic lesions was also evident on gross examination of the pancreas, where large nodules were clearly evident within the expanded parenchyma (Supplementary Fig. S3).We did not observe cystic lesions in Pdx1-Cre;Smad4 lox/lox animals but did note focal IPMA in two of six older Pdx1-Cre;Kras G12D/+ mice that was not associated with the loss of Smad4 based on immunohistochemical staining (ages 24 and 30 weeks; Fig. 2M-P and data not shown).Pancreata from these mice also showed ductulo-insular complex involvement (Fig. 2Q and R), indicating that these structures may be derived from Smad4-independent changes occurring downstream of Kras G12D expression.
Immunohistochemical characterization of Pdx1-Cre;-Kras G12D/+ ;Smad4 lox/lox mice.Further characterization of Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox and Pdx1-Cre;Smad4 lox/lox animals at 33 weeks of age showed that Smad4 expression was lost in ductal epithelial cells (Fig. 3, top row, arrowheads) and in acinar cells, whereas high-level expression remained evident in the stroma (two right columns, arrows).Because Pdx1 is expressed in pancreatic progenitor cells during development, Pdx1-Cre would not be expected to delete Smad4 within the stromal compartment in Pdx1-Cre;Smad4 lox/lox mice.Ductal epithelia that lacked Smad4 showed nuclear phospho-Smad2 expression, which indicates that certain arms of the TGFh signaling pathway remain intact in the absence of Smad4 (Fig. 3).Ductal lesions were composed of epithelial cells, as determined by CK19 positivity (see Fig. 3, arrowheads) and expressed mucin based on Alcian blue staining (Fig. 3).Ki-67 staining of the ductal epithelium showed that Kras G12D expression enhanced ductal proliferation f2-fold in histologically normal ducts (as compared with Pdx1-Cre control ducts) and was significantly increased in high-grade mPanIN lesions as previously described (Supplementary Table S1; Supplementary Fig. S4; ref. 13).The loss of Smad4 had a modest, but statistically significant, effect in promotion of Kras G12D -associated ductal proliferation in early-stage mPanIN-1A lesions (P < 0.05; Table 2).Higher frequencies of proliferating ductal cells in more advanced mPanIN lacking Smad4 were noted but were not statistically significant.There was no increase in Ki-67 staining in the apparently normal ductal epithelium of Pdx1-Cre;Smad4 lox/lox animals compared with Pdx1-Cre control mice.
Loss of Smad4 in the context of Kras G12D promotes progression to PDAC without the loss of p16 Ink4A or p53.Because human PDAC is associated with the loss of the p16 INK4A and p53 tumor suppressors in f90% and 75% of cases, respectively, we analyzed four independent pancreatic ductal epithelial cell lines that were clonally derived from each of two moribund Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice to determine if histopathologic progression might be due to the loss of either p16 Ink4A and/or p53 function (see Supplementary Fig. S5 for representative morphology of murine ductal cell lines compared with a human ductal epithelial cell line and for CK19 expression).Western blot analysis using an equivalent amount of protein from a pancreatic cell line derived from a Pdx1-Cre;Kras G12D/+ animal (Fig. 4, Ras) or from representative early-passage cell lines clonally isolated from Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice (mouse nos.162 and 537, Table 1) showed that both p53 and the p53 target gene, p21/Waf1/Cip1, were highly induced by g-irradiation (10 Gy using a cobalt source).Sequencing of a PCR product amplified from the p53 locus also revealed that no point mutations had occurred within the p53 coding sequences (data not shown).These results suggest that the p53 signaling pathway remained intact in the presence of the Kras G12D and Smad4 lox/lox mutations.Because p16 Ink4A is known to regulate cdk4 and cdk6 phosphorylation of the Rb protein, we analyzed for expression of both p16 Ink4A and phosphorylated Rb (pRb) in Kras G12D ;Smad4 lox/lox mutant cells.Figure 4 shows that both phosphorylated forms of Rb were present in Kras G12D ;Smad4 lox/lox mutant cells as was p16 Ink4A .These results indicate that significant acceleration of pancreatic neoplasia in Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice can occur in the presence of p16 Ink4A and p53.Previous studies have shown that TGFh signaling via a Smad4-dependent pathway can induce transcription of both p15 Ink4B and p21/Waf1 (40)(41)(42); thus, we tested whether p15 Ink4B expression was affected in Smad4-deficient cells.Western blot analysis showed that p15 Ink4B was expressed at relatively high levels in Smad4-deficient cell lines as compared with the line derived from a Pdx1-Cre;Kras G12D/+ animal, which indicates that other factors can maintain p15 Ink4B expression in the absence of Smad4 within the ductal epithelium.
Pdx1-Cre;Kras G12D ;Smad4 lox/lox mice exhibit squamous cell carcinoma in the esophagus/stomach and polypoid lesion formation in the duodenum.One limitation of the Pdx1-Cre transgenic system is expression of Pdx1 in regions outside of the pancreas during embryogenesis, including cells that give rise to the antral stomach and rostral duodenum (34).Although we noted no gross abnormalities in the pancreas of Pdx1-Cre;Smad4 lox/lox mice up to 6 months of age, all Pdx1-Cre;Smad4 lox/lox mice examined (n = 10) displayed significant polyp formation and had occasional cellular atypia in the duodenum that was not observed in age-matched Pdx1-Cre control or in Pdx1-Cre;Kras G12D/+ animals (Fig. 5, compare A and B with C and D).Polyps in Pdx1-Cre; Smad4 lox/lox mice seemed to cause moderate obstruction and occupied as much as 50% to 70% of the intestinal lumen.This level of obstruction was not severe enough to cause significant weight loss in any of the Pdx1-Cre;Smad4 lox/lox mice that were examined up to 14 months of age (data not shown).Cells lining the duodenum of age-matched Pdx1-Cre;Kras G12D/+ animals (n = 5) were mildly hyperplastic and showed cystic changes compared with control animals (Fig. 5B and data not shown).Hyperplastic mucosae in the duodenum of Pdx1-Cre;Kras G12D/+ animals were previously noted by others (13).In Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice, more pronounced neoplastic polypoid lesions were present in the duodenum along with evidence of carcinoma in situ by 15 weeks of age (Table 1, Fig. 5E and F).Gross examination frequently showed an enlarged duodenum (Fig. 5G, arrow) along with expansion of pancreatic tissue (asterisk).Analysis of the stomach and esophagus in moribund Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice showed that 4 of 16 animals had squamous cell carcinoma that arose either from the stomach or from the esophagus as early as 6 weeks of age (Table 1; Fig. 5K-M).In two instances, carcinoma was noted to extend into the muscularis propria of the esophagus (Table 1, mouse nos.62 and 490).Analysis of Pdx1-Cre;Kras G12D/+ mice (n = 5) showed more subtle polypoid and hyperplastic foveolar cell changes in both the stomach and esophagus (Fig. 5I and data not shown).Characterization of Pdx1-Cre;Smad4 lox/lox animals at 30 weeks of age revealed polypoid lesions in the stomach of two of four animals (Fig. 5J).The abnormal changes associated with the stomach/esophagus in Pdx1-Cre;Smad4 lox/lox animals did not result in any death in animals observed beyond 14 months of age (n = 8).The complex phenotypes arising in moribund Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice often made it difficult to ascertain the precise cause of death, which occurred with a mean survival of 16 weeks (n = 16; Table 1; Supplementary Fig. S6 for Kaplan-Meier survival curve).However, it was clear that more than half of all moribund Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox animals had very little normal pancreatic tissue, which would have contributed significantly to the pathology observed in the animals, and other mice clearly showed evidence of advanced carcinomas/sarcomas that in all likelihood caused the death of the animals.

Discussion
Homozygous deletions and intragenic mutations at the SMAD4 locus on human chromosome 18q21.1 occur predominantly in pancreatic cancer and at reduced frequencies in colorectal and gastric cancers (22,25,43,44).The specific association of loss of SMAD4 with defined cancer types supports the importance of TGFh signaling in suppression of these malignancies.In PDAC, loss of SMAD4 occurs late in disease progression at a stage typically associated with invasive disease and subsequent to loss of the tumor suppressor genes, p16 INK4A and p53, and activating mutations in KRAS (23).In our studies, homozygous deletion of Smad4 in the context of an activating Kras G12D mutation significantly accelerated neoplastic changes in the ductal epithelium that resembled both mPanIN and IPMN of the gastric/foveolar type and promoted active fibrosis that was associated with significant acinar cell loss.These changes occurred without the loss of p53 or p16 Ink4A (Fig. 4).Interestingly, the loss of Smad4 only seemed to promote disease progression in the presence of the Kras G12D mutation because we observed no abnormal pancreatic histology or illness in 23 Pdx1-Cre;Smad4 lox/lox animals that were Figure 2. Invasive PDAC and other histologic features in Pdx1-Cre;Kras G12D/+ ; Smad4 lox/lox pancreas.A to C, localized development of invasive PDAC that was moderately differentiated (asterisk ) was observed in 33-wk-old (A and B ; mouse 162; Â200 and Â600) and 25-wk-old (C ; mouse 7; Â400) Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox animals.D and E, sarcomatoid lesions in a 23-week-old double-mutant pancreas (D ; mouse 239; Â400) that also exhibited liver metastasis (E; white arrowhead; Â400; Li, liver).F, adenosquamous carcinoma (arrowheads ) in a 6-wk-old animal (mouse 484).G, IPMA with duct ectasia and pancreatitis (arrowheads ) in a representative 6-wk-old Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mouse (mouse 490; Â100).H and I, IPMN of the gastric-foveolar type showing endocrine cells within the papilla (arrowhead; mouse 7; Â200 and Â400).J, IPMN with rare papilla with acinar cells at the tip (arrowhead; mouse 537; Â400).K and L, IPMN involving the branch ducts with papillae formation in a 14-wk-old animal (mouse 537; Â100 and Â400).M and N, 24-wk-old Pdx1-Cre;Kras G12D/+ animal with evidence of IPMN (arrowhead; Â100 and Â400).O and P, immunohistochemical staining showing Smad4 expression in preinvasive ductal epithelium in IPMN observed in Pdx1-Cre;Kras G12D/+ animal (O) and the lack of Smad4 expression in a Pdx1-Cre;Smad4 lox/lox control ductal epithelium (P ; see arrowheads ; Â400).Q and R, ductulo-insular structure present within Pdx1-Cre;Kras G12D/+ mouse (Â100 and Â400).

Cancer Research
Cancer Res 2007; 67: (17).September 1, 2007 analyzed up to 14 months of age.This indicates that Smad4 is dispensible for normal pancreatic development but is critical for at least partial suppression of multiple Kras G12D -dependent diseaseassociated phenotypes.
Previous studies using the Pdx1-Cre transgenic model in mice have shown that homozygous loss of either p53 or p16 Ink4A in conjunction with the Kras G12D mutation results in the development of lethal pancreatic malignancies by 8 and 18 weeks of age, respectively (21).Deletion of both p16 Ink4A and p19 Arf (in the presence of wild-type p53) accelerated lethality to 8 to 11 weeks, which indicates that both the p53 and pRb pathways are critical for suppression of PDAC progression (14,21).In the context of Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice, death occurred with a mean latency of 16 weeks, although death was likely due to tumor formation in the gastric epithelium and not to pancreatic abnormalities in f25% of cases (Table 1).However, all moribund animals showed significant histopathologic abnormalities affecting both the pancreatic tissue and the stroma at the time of sacrifice that were substantially more progressed than age-matched Pdx1-Cre;Kras G12D/+ mice (Fig. 2B and Supplementary Fig. S1).Although Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice progressed to locally invasive PDAC (Fig. 2A-C), their less aggressive pancreatic phenotype compared with Pdx1-Cre;Kras G12D animals with homozygous deletions in p53 or p16 Ink4A supports observations in human disease where these mutations (particularly loss of p16 INK4A expression) occur in association with SMAD4 inactivation (11).The observation that p53, p16 Ink4A , and p15 Ink4B continue to be expressed at apparently wild-type levels in ductal epithelial cell lines cultured from moribund Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice might explain the less aggressive disease presentation, NOTE: Percentage of Ki-67-positive ductal cells within preinvasive ductal lesions in mice of the defined genotypes.The total number of ductal cells counted from at least three independent mice per genotype is shown in parentheses.Statistically significant differences in the frequencies of proliferating ductal cells were noted in mPanIN-1A ducts typed from Pdx1-Cre;Kras G12D/+ animals versus Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice (P < 0.05) but not at other mPanIN stages, although all stages showed a trend toward increased proliferation with loss of Smad4.especially with respect to metastasis, as compared with Kras G12Dexpressing animals that lack p53 or p16 Ink4A /p19 Arf .In contrast to previous murine studies where mPanIN was observed in association with Kras G12D expression, the loss of Smad4 in the context of the Kras G12D mutation resulted in the development of preinvasive IPMN-like lesions that were evident in the majority (14 of 16) of both young and older Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice (Fig. 2G-L; Table 1).These observations contrast with human IPMN cases, which exhibit activated KRAS expression but typically not the loss of SMAD4 (32,33).This suggests that development of IPMN may occur through distinct genetic pathways in mice as compared with humans.Alternatively, IPMN-like changes associated with Smad4 loss in mice may be occurring due to the continued presence of p16 Ink4A and/or p53, which may be altering the normal genetic progression pathways associated with development of IPMN and mPanIN.This suggestion is supported by a recent study from the DePinho group, where they elegantly show that loss of p16 Ink4A / p19 Arf results in a decreased incidence of IPMN in the context of either Pdx1-Cre;Kras G12D/+ ;Ink4a/Arf lox/lox ;Smad4 lox/lox or Ptfa1-Cre;Kras G12D/+ ;Ink4a/Arf lox/lox ;Smad4 lox/lox mice (45).With respect to both the subtype and incidence of IPMN and to overall survival and pancreatic histopathology, our observations of Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice are consistent with this recent study.Significantly, we also noted rare focal, gastric-type IPMN in two of six older Pdx1-Cre;Kras G12D/+ mice that was not associated with the loss of Smad4 (Fig. 2M-P), although the appearance of IPMN was extremely rare in Pdx1-Cre;Kras G12D/+ animals.This suggests that IPMN in mice may develop by a Smad4-independent pathway, as is presumably the case for human pancreatic tumors exhibiting IPMN.In another study, IPMN was not noted in the context of Ptfa1-Cre;Kras G12D ;Tgfbr2 lox/lox mice, where rapid lethality and PDAC arise in the context of mPanIN and desmoplasia (46).The contrasting ductal phenotypes associated with Kras G12D expression and the loss of either Smad4 or the type II TGFh receptor could reflect a loss of responsiveness to other receptor signaling pathways that use Smad4, like activin or BMP.Alternatively, these differences may highlight the importance of Smad4-independent pathways in rapid tumor promotion in the Ptfa1-Cre;Kras G12D ;Tgfbr2 lox/lox model.Interestingly, mucinous cystic neoplasm and not IPMN was observed in another very recent study of Ptfa1-Cre;Kras G12D ;Smad4 lox/+ mice (47).Differences between our observations and this study could be due to modifying effects of the genetic background, which was more closely C57BL/6 in our analysis.In addition, development of mucinous cystic neoplasm may be favored over IPMN in the context of Smad4 haploinsufficiency that progresses through loss of the wild-type Smad4 allele, whereas IPMN may develop in association with rapid and complete loss of Smad4.Because another study noted IPMN and mPanIN in the context of Ptfa1-Cre (45), it is unlikely that differences in cell type-or stage-specific expression of Cre from the Pdx1 or Ptfa1 transgene are causing this difference in neoplastic presentation.The complete absence of ovarian-type stroma and the lack of progesterone and estrogen receptor positivity, as well as the clear involvement of cystic lesions throughout the pancreas in association with the branch (and sometimes the main) pancreatic ducts (see Supplementary Fig. S1, mouse nos.490 and 523 as examples), strongly rule out mucinous cystic neoplasm in the context of this study.
It is presently unclear what drives the significant histopathologic changes that are observed in Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice.One prominent feature of Smad4 loss is the marked expansion of reactive stroma and enhanced acinar cell loss that may have been due, at least in part, to acinar-to-ductal metaplasia stimulated by Kras G12D expression in acinar tissue, as noted in previous transgenic and knock-in models (Fig. 1D; refs.13,48,49).However, acinar-to-ductal metaplasia occurring in Pdx1-Cre; Kras G12D/+ ;Smad4 lox/lox mice was much more focal and less pronounced than in models where Kras G12D was expressed using acinar-specific transgenes or Cre-deleter strains.The of protein from a ductal cell line isolated from the pancreas of a Pdx1-Cre; Kras G12D/+ mouse (Ras ) or from independent clonal cell lines isolated from two Pdx1-Cre;Kras G12D/+ ;Smad4 lox/lox mice (mouse nos.162 and 537) was analyzed for expression of Smad4, p15Ink4B, p16Ink4A, pRb, p53, and p21.Cell lines from Pdx1-Cre;Kras G12D/+ ; Smad4 lox/lox mice (four lines per animal, with not all lines shown) were either untreated or g-irradiated using 10 Gy from a cobalt source (R lanes ).Lane +, positive controls recommended by the antibody distributor (see Materials and Methods).