jf6b02782_si_001.pdf (431.29 kB)
Stability and Bioavailability of Lentztrehaloses A, B, and C as Replacements for Trehalose
journal contribution
posted on 2016-09-05, 00:00 authored by Shun-ichi Wada, Ryuichi Sawa, Shun-ichi Ohba, Chigusa Hayashi, Maya Umekita, Yuko Shibuya, Kiyoko Iijima, Fumiki Iwanami, Masayuki IgarashiTrehalose is widely
used as a sweetener, humectant, and stabilizer,
but is ubiquitously degraded by the enzyme trehalase expressed in
a broad variety of organisms. The stability of the new trehalose analogues
lentztrehaloses A, B, and C in microbial and mammalian cell cultures
and their pharmacokinetics in mice were analyzed to evaluate their
potential as successors of trehalose. Among the 12 species of microbes
and 2 cancer cell lines tested, 7 digested trehalose, whereas no definitive
digestion of the lentztrehaloses was observed in any of them. When
orally administered to mice (0.5 g/kg), trehalose was not clearly
detected in blood and urine and only slightly detected in feces. However,
lentztrehaloses were detected in blood at >1 μg/mL over several
hours and were eventually excreted in feces and urine. These results
indicate that lentztrehaloses may potentially replace trehalose as
nonperishable materials and drug candidates with better bioavailabilities.