Sequence alignments of individual EC repeats of PCDH24.
Multiple sequence alignments comparing each EC repeat and MAD10 of PCDH24 from 13 different species. Each alignment is colored by percent identity, with white being the lowest percent identity and dark blue being the highest. Sites of N-linked (blue in human, green in mouse) and O-linked (purple in human, red in mouse) glycosylation are denoted by a colored circle. An asterisk (*) indicates sites Y67 and Y71 mutated in binding assays. Secondary structure elements observed in the crystal structures of hs PCDH24 EC1-2 and mm PCDH24 EC1-3 are illustrated below the respective repeats. Residues at the antiparallel homophilic hs PCDH24 EC1-2 II interface (>30% buried surface area) are denoted by a light blue bar. Calcium-binding motifs are indicated above the sequences, which are numbered according to the human protein. Species are abbreviated as follows: Homo sapiens (Hs), Mus musculus (Mm), Sus scrofa (Ss), Gallus gallus (Gg), Aptenodytes forsteri (Af), Parus major (Pm), Anolis carolinensis (Ac), Crocodylus porosus (Cp), Thamnophis elegans (Te), Danio rerio (Dr), Oryzias melastigma (Om), Mastacembelus armatus (Ma), and Xenopus tropicalis (Xt). Species were chosen based on sequence availability, coverage of entire extracellular domain, and taxonomical diversity. Accession numbers and species can be found in S2 Table. EC, extracellular cadherin; PCDH24, protocadherin-24.