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journal contribution

posted on 2022-05-06, 17:33 authored by Emily K. Matozel, Stephen Parziale, Allen C. Price

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A programmable DNA roadblock system using dCas9 and multivalent target sites

PLOS ONE

Categories

Biophysics
Biochemistry
Genetics
Molecular Biology
Biological Sciences not elsewhere classified
Infectious Diseases

Keywords

restriction endonuclease ndei provide full protocols programmability allows us cleavage deficient mutant verify complete binding variable specific sites internal restriction sites synthetic gene intended protein binds dna create binding sites dna binding proteins protein roadblock forms div >< p dna binding target sites gene expression vitro </multivalency —introduce non experimental methods dna roadblocks

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