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posted on 2022-05-06, 17:33 authored by Emily K. Matozel, Stephen Parziale, Allen C. Price

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A programmable DNA roadblock system using dCas9 and multivalent target sites
PLOS ONEPLOS ONE

Categories

  • Biophysics
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Biological Sciences not elsewhere classified
  • Infectious Diseases

Keywords

restriction endonuclease ndeiprovide full protocolsprogrammability allows uscleavage deficient mutantverify complete bindingvariable specific sitesinternal restriction sitessynthetic gene intendedprotein binds dnacreate binding sitesdna binding proteinsprotein roadblock formsdiv >< pdna bindingtarget sitesgene expressionvitro </multivalency —introduce nonexperimental methodsdna roadblocks

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CC BY 4.0CC BY 4.0

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