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Ratiometric Temperature Sensing with Semiconducting Polymer Dots

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journal contribution
posted on 01.06.2011, 00:00 by Fangmao Ye, Changfeng Wu, Yuhui Jin, Yang-Hsiang Chan, Xuanjun Zhang, Daniel T. Chiu
This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dyeRhodamine B (RhB), whose emission intensity decreases with increasing temperaturewithin the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.