Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B<sub>1</sub> and amantadine
journal contribution
posted on 2022-06-14, 21:40 authored by Kezhuo Zhang, Huadong Cai, Min Lu, Daixian Wei, Jiaqi Yin, Nengshui Ding, Weihua Lai, Juan Peng<p>A bispecific monoclonal antibody (BsMAb) that can simultaneously recognize aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and amantadine (AMD) was prepared. Quantum dot nanobead immunochromatographic assay (QB-ICA) based on the BsMAb was developed for the simultaneous detection of AFB<sub>1</sub> and AMD in four feed samples (suckling pig feed, piglet feed, sow feed, and compound feed for laying ducks). Under optimal experimental conditions, the LOD values of QB-ICA for AFB<sub>1</sub> in four feed samples were 0.188, 0.221, 0.233, and 0.306 μg/kg, respectively. The LOD values of QB-ICA for AMD in the same feed samples were 0.125, 0.187, 0.076, and 0.191 μg/kg, respectively. The recovery rates of QB-ICA for AFB<sub>1</sub> and AMD ranged from 91.55% to 150.87% and from 91.62% to 138.42%, respectively. The variation coefficients were all less than 15%. The proposed QB-ICA had high reliability and specificity for the simultaneous detection of AFB<sub>1</sub> and AMD, providing a platform for simultaneous detection of hazardous substances.</p>
