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Protein Nanosheet Mechanics Controls Cell Adhesion and Expansion on Low-Viscosity Liquids

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journal contribution
posted on 2018-02-07, 00:00 authored by Dexu Kong, William Megone, Khai D. Q. Nguyen, Stefania Di Cio, Madeleine Ramstedt, Julien E. Gautrot
Adherent cell culture typically requires cell spreading at the surface of solid substrates to sustain the formation of stable focal adhesions and assembly of a contractile cytoskeleton. However, a few reports have demonstrated that cell culture is possible on liquid substrates such as silicone and fluorinated oils, even displaying very low viscosities (0.77 cSt). Such behavior is surprising as low viscosity liquids are thought to relax much too fast (