New steroid produced by Periconia pseudobyssoides K5 isolated from Toona sureni (Meliaceae) and its heme polymerization inhibition activity

Azhari, Azmi; Naini, Al Arofatus; Harneti, Desi; Wulandari, Asri Peni; Mulyani, Yeni; Purbaya, Sari; Sari, Aprilia Permata; Pratama, Galih Bayu; Anwar, Risyandi; Fajar, Mohammad; Abdullah, Fajar Fauzi; Farabi, Kindi; Supratman, Unang

doi:10.6084/m9.figshare.22560285.v1

New steroid produced by Periconia pseudobyssoides K5 isolated from Toona sureni (Meliaceae) and its heme polymerization inhibition activity

journal contribution

posted on 2023-04-05, 10:46 authored by Azmi Azhari, Al Arofatus Naini, Desi Harneti, Asri Peni Wulandari, Yeni Mulyani, Sari Purbaya, Aprilia Permata Sari, Galih Bayu Pratama, Risyandi Anwar, Mohammad Fajar, Fajar Fauzi Abdullah, Kindi Farabi, Unang Supratman

A new ergostane-type steroid named (22E)-3α,6α,9α-ergosta-7,22-diene-3,6,9-triol (1), along with six known steroids 5α,8α-epidioxy-24-ethyl-cholest-6-en-3β-ol (2), ergosterol-5,8-peroxide (3), cerevisterol (4), isocyathisterol (5), 6β-hydroxystigmast-4-en-3-one (6), 6β-hydroxy-4-campesten-3-one (7), were isolated from the fermented unpolished rice media by Periconia pseudobyssoides K5 (Periconiaceae), an endophytic fungus from medicinal plant Toona sureni (Meliaceae). The fermentation takes at 28 ± 2 °C for 30 days. The structure of new steroid (1) was elucidated by extensive spectroscopic measurements (IR, HR-ESI-TOFMS, and 1D and 2D NMR) analyses. The isolated compounds (1–7) were evaluated for heme polymerization inhibition assay (HPIA). The IC₅₀ HPIA value of 1 is 8.24 ± 0.03 mg/ml.