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Download fileNanoparticle–Drug Bioconjugate as Dual Functional Affinity Ligand for Rapid Point-of-Care Detection of Endotoxin in Water and Serum
journal contribution
posted on 2015-11-03, 00:00 authored by Prasanta Kalita, Anshuman Dasgupta, Venkataraman Sritharan, Shalini GuptaEndotoxin
or lipopolysaccharide (LPS) is a major constituent of
the Gram-negative bacterial cell wall that causes a life-threatening
disorder called septicemia resulting from the unregulated activation
of the innate immune system. We demonstrate a simple and robust drug-assisted
dot blot bioassay for endotoxin detection that can be used right by
the critically ill patients’ bedside. Target LPS molecules
are trapped from serum or water on glass substrates via long-chain
alkyls and tagged with reporter gold nanoparticles (NPs) preconjugated
to an antibiotic drug called polymyxin B sulfate (PMB). A post-silver-enhancement
step enables signal visibility to the bare eye over a wide and clinically
relevant concentration range of 50 fg/mL–50 ng/mL, allowing
effortless diagnosis of sepsis at various stages, from early sepsis
to septic shock.
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RapidsepticemiasepsisDual Functional Affinity LigandEndotoxinpreconjugatedrightcell wallserumblotdiagnosisPMBbioassayNPlipopolysaccharideantibiotic drugtarget LPS moleculesactivationsignal visibilityfgconstituentSerumEndotoxinglass substratesBioconjugateendotoxin detectionpolymyxin B sulfateDetectionalkylbedsideNanoparticlereporter gold nanoparticlesconcentration rangedisorder