Mitsi et al. , Biol. Lett. (2019), Supplementary Materials

Supplementary Materials of "A global metabarcoding analysis expands molecular diversity of Platyhelminthes and reveals novel early-branching clades"

The reference tree used for the phylogenetic placement is the Supp7 and the alignment of the reference tree in phylip format is the Supp8. The alignment of queries on the reference tree produced by the software papara that was used for the phylogenetic placement can be found in Supp4. We have produced 2 jplace placement files using different software, one with EPA that can be found as Supp1 and one with pplacer that can be found as Supp6. We compared our jplace files using genesis toolkit and the results can be found at Supp5. The best-hit placement tree of Fig 1C is the Supp2. The maximum likelihood tree of the new clades of Fig 2B is the Supp3.