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MOESM4 of Characterization of a pathway-specific activator of milbemycin biosynthesis and improved milbemycin production by its overexpression in Streptomyces bingchenggensis

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posted on 2016-09-07, 05:00 authored by Yanyan Zhang, Hairong He, Hui Liu, Haiyan Wang, Xiangjing Wang, Wensheng Xiang
Additional file 4: Figure S4. Effect of site-directed mutation in the AAA domain of MilR on the transcription of milA2, milC, milA3 and milA1. Transcriptional analysis of milA2, milC, milA3 and milA1 were performed by semiquantitative RT-PCR. MilR:ΔmilR/milR, 31A:ΔmilR/G31A, 32A:ΔmilR/G32A, 34A:ΔmilR/G34A, 36A:ΔmilR/G36A, 37A:ΔmilR/K37A, 37R:ΔmilR/K37R, 38A:ΔmilR/S38A, 122A:ΔmilR/D122A, 123A:ΔmilR/D123A.


National Natural Science Foundation of China