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MOESM3 of HIV latency reversing agents act through Tat post translational modifications

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posted on 11.05.2018, 05:00 by Georges Khoury, Talia Mota, Shuang Li, Carolin Tumpach, Michelle Lee, Jonathan Jacobson, Leigh Harty, Jenny Anderson, Sharon Lewin, Damian Purcell
Additional file 3: Figure S3. Cellular and HIV RNA levels following JQ1 treatment. A. Absolute quantification of RPP30, IPO8 and TBP cellular mRNAs (copies/μl) were performed using total RNAs derived from transfected HEK293T cells with the pLTR.gp140/EGFP.Rev∆38/DsRed splicing reporter in the absence and presence of 100 ng of pTat101 (AD8)-Flag expression plasmid and treated with JQ1 (1 μM) or DMSO diluent control. B. HIV unspliced (US), spliced (D4-A7) and all viral RNA expression levels (copies/ul) were quantified by droplet digital PCR (ddPCR) and normalized over the 3 reference genes. Comparisons of each condition to DMSO were made using a paired T test. Only statistically significant comparisons are shown *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The black lines represent the mean ± SEM (n = 4)

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