MOESM3 of Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs

Additional file 3: Figure S3. Saccharification of Avicel PH-101 in the presence of sulfite under aerobic (blue bars) or anaerobic (red bars) conditions, in the presence or absence of 1 mM ascorbic acid (+/− AscA). The left panels show glucan conversion (as a percentage of theoretical glucan conversion) and the right panels show concentration of Glc4gemGlc, at two time points. Enzymatic hydrolysis was carried out with Cellic® CTec3 (a, b) and Celluclast:Novozym 188 (mixed at 5:1 ratio, w/w) (c, d) at 8 mg/g glucan total protein loading in reaction mixtures containing 5 % DM in 50 mM sodium acetate pH 5.0 that were incubated at 50 °C. Data for similar reactions without sulfite are presented in Fig. 1. The data points represent the average value of three independent experiments with one technical replicate per experiment. The error bars represent standard deviations of the three independent experiments. The statistical significance of differences in the 48 hour saccharification yields was analyzed using two-way ANOVA with Tukey’s post hoc test (95% confidence interval), and is indicated as follows: *, p<0.05; **, p<0.01; ***, p<0.001.