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MOESM2 of Identification of Plasmodium falciparum specific translation inhibitors from the MMV Malaria Box using a high throughput in vitro translation screen

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posted on 2016-03-17, 05:00 authored by Vida Ahyong, Christine Sheridan, Kristoffer Leon, Jessica Witchley, Jonathan Diep, Joseph DeRisi
Additional file 2: Figure S2. Standard high throughput assay parameters. A. Seven different lysates were assayed in the presence or absence of the luciferase reporter mRNA to calculate the signal-to-noise ratio. B. A histogram showing all DMSO controls from the screen. The percent coefficient of variation was calculated from these controls. C. Calculating the Z’ factor from a single lysate that was assayed in the presence or absence of the luciferase reporter mRNA using either the raw or normalized controls. For this calculation, the positive controls are in the absence of mRNA and the negative controls contain luciferase mRNA.

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Howard Hughes Medical Institute

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