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MOESM2 of Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs

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posted on 11.07.2017 by Piotr Chylenski, Dejan Petrović, Gerdt Müller, Marie Dahlström, Oskar Bengtsson, Martin Lersch, Matti Siika-aho, Svein Horn, Vincent Eijsink
Additional file 2: Figure S2. Saccharification of sulfite-pretreated Norway spruce without addition of l-cysteine hydrochloride monohydrate (0.025 % w/v) in the anaerobic reactions, in the absence (a, b) or presence (c, d) of sulfite under aerobic (blue bars) or anaerobic (red bars) conditions, in the presence or absence of 1 mM ascorbic acid (+/− AscA). The left panels show glucan conversion (as a percentage of theoretical glucan conversion) and the right panels show the concentration of Glc4gemGlc, at two time points. Enzymatic hydrolysis was carried out with Cellic® CTec3 at 8 mg/g glucan total protein loading in reaction mixtures containing 5% DM in 50 mM sodium acetate pH 5.0 that were incubated at 50 °C. Reactions with sulfite (lower panels) contained 1000 ppm of sulfite ions added as a sodium sulfite (12.5 mM). The data points represent the average value of three independent experiments with one technical replicate per experiment. The error bars represent standard deviations of the three independent experiments. The statistical significance of differences in the 48 hour saccharification yields was analyzed using two-way ANOVA with Tukey’s post hoc test (95% confidence interval), and is indicated as follows: *, p<0.05; **, p<0.01; ***, p<0.001.

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Research Council of Norway

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