MOESM2 of Development of a liquid chromatography high resolution mass spectrometry method for the quantitation of viral envelope glycoprotein in Ebola virus-like particle vaccine preparations
journal contributionposted on 05.09.2016, 05:00 by Lisa Cazares, Michael Ward, Ernst Brueggemann, Tara Kenny, Paul Demond, Christopher Mahone, Karen Martins, Jonathan Nuss, Trevor Glaros, Sina Bavari
Additional file 2: Figure S1. XIC profiles of each peptide charge state used in the quantitation showing the retention time alignment at 4 ppm. Data was taken from eVLP lot ‘B’ dilution ‘2’ replicate. The 4 profiles from each peptide are ordered from AQUA 2+ (H 2+), analyte 2+ (L 2+), AQUA 3+ (H 3+) and analyte 3+ (L 3+). A) Represents the SVG and SVGR peptide Set 2, B) is the SEE and IRSEE peptide Set 1 and C) shows the XIC profiles of the deamidated SVG and SVGR analyte peptides. XIC values were acquired with at least 7 data points sampled across the elution profile.