MOESM1 of Hydrogen sulfide decreases high glucose/palmitate-induced autophagy in endothelial cells by the Nrf2-ROS-AMPK signaling pathway

Additional file 1: Figure S1. NaHS effected on autophagy and apoptosis of RAECs with the treatment of HG and Pal. Figure S2. RAECs were treated with HG and Pal in the presence of autophagy activator (rapamycin) to determine the expression of LC3II/I and Atg7 by western blot analysis. Figure S3. Atg7 siRNA reducing the expression of LC I/II in RAECs treated with HG and Pal. Figure S4. RAECs were treated with HG and Pal and NAHS in presence of AMPK catalyst (AICAR) to determine the expression of mitophage associated proteins (Parkin and Beclin 1) by western blot analysis. Figure S5. The cell death of RAECs treated by HG and Pal with 3-MA and Atg7 siRNA was measured by Hoechst 33342/PI staining.