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MOESM1 of Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs

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posted on 11.07.2017 by Piotr Chylenski, Dejan Petrović, Gerdt Müller, Marie Dahlström, Oskar Bengtsson, Martin Lersch, Matti Siika-aho, Svein Horn, Vincent Eijsink
Additional file 1: Figure S1. Saccharification of pretreated Norway spruce in the absence (a, b) or presence (c, d) of sulfite under aerobic (blue bars) or anaerobic (red bars) conditions in the presence or absence of 1 mM gallic acid (+/− GA; left panels) or reduced glutathione (+/− RG; right panels). Enzymatic hydrolysis was carried out with Cellic® CTec3 at 8 mg/g glucan total protein loading in reaction mixtures containing 5% DM that were incubated in 50 mM sodium acetate pH 5.0, at 50 °C. Reactions with sulfite (lower panels) contained 1000 ppm of sulfite ions added as a sodium sulfite (12.5 mM). The data points represent the average value of three independent experiments with one technical replicate per experiment. The error bars represent standard deviations of the three independent experiments. The statistical significance of differences in the 48 hour saccharification yields was analyzed using two-way ANOVA with Tukey’s post hoc test (95% confidence interval), and is indicated as follows: *, p<0.05; **, p<0.01; ***, p<0.001.

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Research Council of Norway

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