MOESM1 of Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation
journal contributionposted on 2019-09-24, 03:37 authored by Patricia Vázquez, Koldo Osoro, Miguel Fernández, Alicia Román-Trufero, Javier Regidor-Cerrillo, Laura Jiménez-Pelayo, Marta García-Sánchez, Silvia Rojo-Montejo, Julio Benavides, Pilar Horcajo, Luis Ortega-Mora
Additional file 1. Materials and methods. Detailed description of the (1) health and reproductive management of heifers and (2) molecular (tissue DNA extraction and PCR and qPCR determinations), cellular (peripheral blood stimulation assays and quantification of IFN-γ) and serological analyses (ELISA and IFAT).